AAAAI Edition, Washington DC  
March 2009  


Charles Darwin was fascinated by the "endless diversity of structure" of Orchids.

In this issue, we focus on the resurgence of new data on the structural diversity of mite allergens published in Nature and the Journal of Molecular Biology. The latter by our own group headed by Dr. Anna Pomes reveals the definitive molecular structures of mite Group 1 allergens, some 30 years after these allergens were first identified.

Our new products for 2009 include recombinant Der p 7 (another important mite allergen), an extended range of LoTox¢â mite, cat, pollen and egg allergens, and three new bead sets for MARIA¢â. Service Partner and Sub-Distributor agreements are available for companies to take advantage of MARIA¢â technology for allergen exposure assessment. We have also added Endotoxin testing to our range of environmental assays.

After a successful meeting of the Indoor Air Quality Association in Ft. Worth TX two weeks ago (see below), this weekend we make the short drive up to Washington DC for the American Academy of Allergy Asthma and Immunology annual meeting. Driving across the Potomac into DC still has an open, colonial feel, like you're driving uptown from the country to pick up supplies.

There's lots on offer in Washington these days, including a resurgence in science. Please stop by our exhibit at AAAAI and pick up a 'cool' Obama card, artwork of Charlottesville-based artist Michelle Price. "Orchids through Darwin's Eyes" is at the Smithsonian National Museum of Natural History and worth a visit.

Welcome to Washington!

in this issue
  • Mite Allergenicity - Exciting News
  • INBIO at AAAAI, Washington DC, March 13-17
  • New Products for 2009
  • MARIA¢â Update
  • Endotoxin Testing
  • Indoor Air Quality News
  • INBIO Training Course on Health Effects of Indoor Allergens - October 09
  • INBIO Video

  • INBIO at AAAAI, Washington DC, March 13-17
    'OBAMA' by Michele Price

    ¨ÏMichele Price

    Please visit our exhibit at booth #730 at the American Academy of Allergy Asthma and Immunology annual meeting in Washington DC from March 13-17.

    Scientists from Indoor Biotechnologies will be making 10 presentations at AAAAI on allergen structure, recombinant allergens, multi-center trial of MARIA¢â and immunoassays for Stachybotrys. [Read more]

    Most of our staff will be spending the weekend in Washington. Come by and pick up CD's with product information and a copy of the INBIO video. And let us know how we can help!

    New Products for 2009

    Recombinant Der p 7 is now available, expressed in Pichia pastoris (Product code RP-DP7-1).

    Our list of LoTox¢â low endotoxin purified allergens has been extended to 13 products, including LoTox¢â mite, cat, pollen and egg allergens. [LoTox¢â Products]. LoTox¢â allergens contain <0.03EU/¥ìg endotoxin and are ideal for cellular studes and for animal models of allergic diseases.

    Check out our range of purified natural and recombinant [food allergens].

    MARIA¢â Update

    Three new beads sets for Alt a 1, Bet v 1, Phl p 5 are available for use in [MARIA¢â], our multiplex allergen array using the Luminex xMAP¢ç system. This expands our multiplexing capability to 11 allergens.
    Results of a multi-center trial comparing data from nine laboratories for allergen measurements on >150 dust samples using the MARIA¢â 8-plex array will be presented at the AAAAI meeting.

    MARIA¢â Service Partner and Sub-Distributor Agreements are available for companies to commercialize this state-of-the-art technology.
    Benefits include:
    • Price discount on MARIA¢â services, kits and DUSTREAM¢â collectors
    • Training for Partner employees
    • "Preferred customer" status
    • Expanded marketing opportunities
    • Higher revenue streams and profitability

    For further details, contact Amy Tsay (

    Endotoxin Testing

    Endotoxin is a marker of exposure to Gram negative bacteria that has effects of the respiratory and immune systems. We are offering endotoxin testing on dust, air and other environmental samples using the Limulus Amebocyte Lysate (LAL) assay. For further details, contact Stephanie Filep (

    Indoor Air Quality News

    The recent IAQA meeting in Fort Worth TX was a great success. We had many interesting and productive discussions with IAQA members.

    Dr. Eva King presented information on allergen exposure assessment at a workshop on "Evaluating Indoor Exposure Sources, with a Focus on Allergy and Asthma Triggers" organized by Carl Grimes. This was followed by a session on "Indoor Allergens - Health Effects, Exposure Assessment and Intervention" with presentations by Dr. King and Dr. Martin Chapman (President, Indoor Biotechnologies). The presentations are available on [pdf].

    There was a genuine interest among IAQA members in introducing home inspections for allergen exposure, especially using MARIA¢â technology. Please contact Dr. King if you would like to follow up on allergen exposure assessments (


    INBIO Training Course on Health Effects of Indoor Allergens - October 09

    Save the Date! Our annual training course on the "Health Effects of Indoor Allergens, Molds and Endotoxin" will be held October 2009, in Charlottesville, Virginia. This will be the 10th year that we have offered this course, which has trained hundreds of health care and IAQ professionals from around the world in allergen exposure assessment [View last year's program].

    • Special discounts for MARIA¢â Service Partners and Distributors.
    • To join the pre-registration list, contact:
    INBIO Video

    This short HD video can be downloaded using Quicktime at:
    Don't have Quicktime? We're also on YouTube:


    Mite Allergenicity -
    Exciting News

    Der f 1 crystal structure

    Two recent papers provide fascinating insights into intrinsic properties of mite allergens that contribute to allergenicity. In a Nature paper in January, Trompette and colleagues at the University of Cincinnati reported that Der p 2 has functional homology to MD-2 (a lipid binding component of Toll-like receptor 4). This allows access of Der p 2 to the innate immune system and may provide an adjuvant effect that enhances IgE responses (Nature 457:585-8, 2009).

    In February, Dr. Anna Pomes at Indoor Biotechnologies and colleagues from the University of Virginia reported the definitive, high resolution structures of the Group 1 mite allergens, Der p 1 and Der f 1, in the 50th anniversary edition of the Journal of Molecular Biology. Analysis of these structures (PDB codes 3F5V and 3D6S, respectively) identified conserved surface residues that are potential IgE epitopes (Chruszcz et al, JMB 386, 520-30, 2009).